Figure 3.
(A) Shown is the expression pattern analysis of wdr31 in zebrafish embryos. wdr31 is ubiquitously expressed before the segmentation stage (two-cell and eight-somite stages [SS]). The expression of wdr31 becomes limited to the otic vesicle and brain region at 24 hours post fertilization (24 hpf). (B) Shown are the cilia of lateral crista (LC) of the otic vesicle, stained with acetylated-tubulin, in WT and wdr31 knockout, generated via CRISPR/Cas9. (C, D) The length of cilia in the LC of the otic vesicle remains unaffected in zebrafish wdr31 knockout, whereas the cilia number is decreased, as shown with the measurement of width of cilia LC of the otic vesicle.