ZFIN Image: Emmerich et al., 2023, Fig. 6

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Figure Caption

Fig. 6

The E3 ubiquitin ligase rnf2 is required for D-Dex enhanced regeneration.

a Volcano plot of DEGs between Mtz only and +Mtz, +D-Dex treated eyes. Red indicates statistically significant upregulation and blue indicates statistically significant downregulation. b qRT-PCR assessment of rnf2 and kcnj13 upregulation in the +Mtz, +D-Dex condition. c Quantification of NTR-YFP-expressing rod cells at 9 dpf to assess changes in regeneration kinetics in +Mtz, +D-Dex larvae following knockdown of rnf2 and kcnj13, n = 54, 22, 24, and 7 from left to right (*p ≤ 0.05). d Representative in vivo confocal images of NTR-rod larval retinas at 9 dpf following +Mtz, +D-Dex treatments in either wildtype control (left) or rnf2 knockdown “crispant” backgrounds. Sample sizes from left to right: 54, 21, 14, and 7.

Acknowledgments

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