Fig. 3

a Process for conjugating Cy5 to PAMAM G4-OH dendrimers (D-Cy5) using Cy5 NHS and borate buffer. b Assay schematic: transgenic zebrafish expressing NTR:rod (yellow) and microglia/macrophages (red) were treated with 2.5 mM Mtz from 5–7 dpf to induce rod cell loss. At 6 dpf, fish were given PC injections of D- Cy-5 (cyan, 2 ng/ul) and imaged using in vivo confocal time series microscopy. c Image stills from representative larva at 0, 2, and 4 hpi with all three channels (top panels) or with the yellow channel removed (bottom panels); insets and orange circles highlight interactions between dendrimers (cyan) and microglia (red). See Supplementary Movie 6 for the time-lapse sequence. d Three (left) and two-channel (right) images still from control non-ablated larva at 2 hpi of D-Cy5; orange circles highlight interactions between dendrimers (cyan) and microglia (red). e Imaris-based quantification of normalized colocalization between microglia (red) and D-Cy5 (cyan) signals in retinas treated ±Mtz (n = 4 larvae per condition), +Mtz values were normalized to paired sibling (−Mtz) control fish imaged on the same day (*p ≤ 0.05).