ZFIN Image: Martin et al., 2023, Figure 5

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Figure Caption

Figure 5

Nkx2.5 induction at 20 hr post-fertilization (hpf) represses pacemaker cardiomyocyte (PC) marker expansion in nr2f1a mutant atria.

(A) Schematic of timeline for heat-shock experiments. (B–I) IHC of representative hearts for Amhc (blue), fgf13a:EGFP (green), and myl7:DsRed2-NLS (red) used to quantify Amhc⁺/fgf13a:EGFP⁺ cardiomyocytes (CMs) marking PCs from wild-type (WT) and nr2f1a mutant embryos with and without Nkx2.5-EGFP. White arrows indicate boundaries of Tg(fgf13a:EGFP) expression. Scale bar indicates 50 μm. (J–K) Quantification of Amhc⁺/fgf13a:EGFP⁺/myl7:DsRed2-NLS⁺ (PCs) and Amhc⁺/fgf13a:EGFP^-/myl7:DsRed2-NLS⁺ cardiomyocytes in the atria with and without Nkx2.5-EGFP induction; 48 hpf WT: Nkx2.5-EGFP^- (n=6), Nkx2.5-EGFP⁺ (n=4); 48 hpf nr2f1a^-/-: Nkx2.5-EGFP^- (n=7), Nkx2.5-EGFP⁺ (n=5); 96 hpf WT: Nkx2.5-EGFP^- (n=4), Nkx2.5-EGFP⁺ (n=4); 96 hpf nr2f1a^-/-: Nkx2.5-EGFP^- (n=7), Nkx2.5-EGFP⁺ (n=4). Differences between WT and nr2f1a^-/- at the different time points were analyzed using Student’s t-test. Error bars indicate s.e.m. *p=0.05–0.001, **p<0.001.

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