ZFIN Image: Martin et al., 2023, Figure 2

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Figure 2

Pacemaker cardiomyocyte (PC) identity expands from the venous pole across the atrium in nr2f1a mutant hearts.

(A–F) IHC for Amhc (blue), Vmhc (red), and fgf13a:EGFP (green). White arrows indicate boundaries of Et(fgf13a:EGFP) expression within the atrium. Number of embryos examined - 48 hr post-fertilization (hpf): wild-type (WT) (n=10), nr2f1a^-/- (n=12); 72 hpf: WT (n=10), nr2f1a^-/- (n=10); 96 hpf: WT (n=7), nr2f1a^-/- (n=10). (G–I) Quantification of Amhc⁺/fgf13a:EGFP^-, Amhc⁺/fgf13a:EGFP⁺, and total Amhc⁺ cardiomyocytes (CMs) using the Tg(myl7:DsRed2-NLS) transgene within the hearts of WT and nr2f1a mutants. Error bars indicate s.e.m.; 48 hpf: WT (n=16), nr2f1a^-/- (n=11); 72 hpf: WT (n=14), nr2f1a^-/- (n=11); 96 hpf: WT (n=15), nr2f1a^-/- (n=12). (J) Schematic for the isolation of cardiomyocytes at 96 hpf using the Tg(myl7:EGFP) transgene. (K) Fold change of marker genes relative to β-actin from real-time quantitative PCR (RT-qPCR) on isolated cardiomyocytes of WT and nr2f1a mutants at 96 hpf. Scale bar indicates 50 μm. Differences between WT and nr2f1a^-/- were analyzed using ANOVA with multiple comparisons. *p=0.05–0.001, **p<0.001.

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