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Fig. 3
Tgs1 down-regulation in zebrafish leads to defects in CaP-MNs. (A) Lateral views of whole-mount embryos immunostained with a synaptotagmin antibody (Znp1) that labels the CaP-MNs. Embryos were untreated or injected with the indicated MOs. Injection of either tgs1 ATG-MO or tgs1 Sp-MO results in truncated or absent motor axons (solid arrowheads) and terminally branched axons (open arrowheads). Scale bar, 25 μm. See also Supplementary Figure S2. (B) Based on overall appearance, CaP-MNs were classified as: normal, short (truncated axonal projection) or absent (axonal atrophy). Based on terminal branching, axons were classified as normal, mild (branching ventral from midline), intermediate (2–3 or more branches at ventral or midline) or severe (>3 branches ventral or dorsal from the midline). Zebrafish larvae injected with 1.5 ng of tgs1 Sp-MO or 2 ng tgs1 ATG-MO displayed CaP-MN defects compared with control (non-targeting) MOs and uninjected fish. Results are percentages from three independent experiments (n = axons analysed; N = animals tested. ****P < 0.0001 χ2 test); n.s. not significant. See also Supplementary Figure S2D.