ZFIN Image: Shoenhard et al., 2022, Fig. 1

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Figure Caption

Fig. 1

CaSR expression after development of behaviorally relevant circuits is sufficient to restore decision making in CaSR mutants

(A) Timeline of zebrafish escape and reorientation circuit development. Both behaviors have emerged by 4 dpf. Letters correspond to panels in this figure. Circles indicate time of heat shock; squares indicate time of behavioral testing.

(B) Fluorescent image of Tg(hsp70:CaSR-EGFP, myl7:GFP) larva without (top) and with (bottom) heat shock. Arrow indicates heart label used for embryo pre-sorting. Scale bar represents 500 um.

(C) Average relative startle bias of 5 days post-fertilization (dpf) sibling and mutant Tg(hsp70:CaSR-EGFP,myl7:GFP) larvae subject to no heat shock or to heat shock at 1 dpf. Blue circles indicate CaSR WT and heterozygous siblings; red squares indicate CaSR homozygous mutants. n.s. incidates p > 0.05.

(D) Average relative startle bias of 5 days post-fertilization (dpf) sibling and mutant Tg(hsp70:CaSR-EGFP,myl7:GFP) larvae subject to no heat shock or to heat shock at 4 dpf. Results are from three experiments. **** incidates p < 0.0001. n.s. incidates p>0.05.

(E) Average relative startle bias of 6 dpf sibling and mutant Tg(hsp70:CaSR-EGFP,myl7:GFP) larvae subject to no heat shock or to heat shock at 5 dpf. No transgene control siblings vs. mutants p = 0.007. No transgene control vs. transgenic mutants, p = 0.0145. n.s. incidates p > 0.05. In (C), (D), and (E), solid lines indicate median; dashed lines indicate quartiles. All p values in (C), (D), and (E) are from Kruskal-Wallis test with Dunn’s multiple comparisons post hoc test.

Acknowledgments

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