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Fig. 4 Characterization of thickness and resolution of LS-FIS. (A) Light-sheet in LS-FIS captured by the bright-field imaging arm with the long pass filter replaced by an appropriate neutral density filter; (B) Intensity along the blue line at the center of light-sheet in (A) is fitted by Gaussian function showing a FWHM of 9.8 μm. (C) Intensity along the red line at halfway away from the center of light-sheet in (A) is fitted by Gaussian function showing a FWHM of 14.1 μm; (D) Raw image of 100 nm fluorescent beads captured by LS-FIS for calibration of the lateral resolution; (E) Intensity profile along the center of the bead marked in (D). The black curve shows the Gaussian fitting to the profile with FWHM of 2.9 μm. Scale bars represent 200 µm for (A) and 150 µm for (D), respectively.