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Fig. 3 (A) Schematic representation of experimental setup and genetic crosses used to obtain wild-type offspring; red cell indicates a germline-specific mutation. (B) Relative mRNA levels of aldh1a2 and aldh1a3 in 24 hpf wild-type embryos from aldh1a2+/+ incrosses (inx) and aldh1a2rec/+ intercrosses (intx). Control expression levels were set at 1. n = 3 biologically independent samples. Data are means ± SD, and a two-tailed Student’s t test was used to calculate P values. Ct values are listed in table S1. (A) was created with BioRender.com.
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