Figure 2

Figure 2

Nanoparticle distribution through retinal layers after intravitreal injection in zebrafish larvae. (a) Representative images of a retinal cross-section of a zebrafish larva IVT injected with Alexa Fluor 488-positive polyacrylamide nanoparticles, analysed at 4 h post-injection (hpi). Hoechst staining allows identification of the retinal layers. In FITC channel, the intense green spots clearly highlight the presence of Alexa Fluor 488-positive nanoparticles in the injected eye. (b) Quantitative evaluation of the distribution of Alexa Fluor 488-positive polyacrylamide nanoparticles in the retinal layers at different time points. n = 15 injected eyes from 3 independent experiments. GCL, ganglion cell layer; INL, inner nuclear layer; PR, photoreceptors; RPE, retinal pigment epithelium. Scale bar: 50 µm.