ZFIN Image: Saraswathy et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Assessment of neuronal progenitors and subtypes in mstnb mutant zebrafish

(A) Experimental timeline to elucidate different steps of adult neurogenesis.

(B) GFP staining in dbx1b:GFP;mstnb^−/− SC sections at 14 dpi. dbx1b:GFP;mstnb^+/+ siblings were used as controls. Dotted ovals delineate central canal edges. Regions in the rectangular boxes are shown at high magnification.

(C and D) The numbers of dbx1b⁺ iNPs (#) were quantified in D SC sections 450 μm rostral to the lesion (C). The proportions of dbx1b⁺ iNPs (%) represent the numbers of dbx1b⁺ iNPs normalized to the total numbers of nuclei for each section (D).

(E) GFP staining in s/c6a5:GFP:GFP;mstnb^−/− SC sections at 14 dpi. slc6a5:GFP:GFP;mstnb^+/+ siblings were used as controls. Dotted ovals delineate central canal edges. Regions in the rectangular boxes are shown at higher magnification.

(F and G) The numbers of glycinergic s/c6a5:GFP⁺ neurons (#) were quantified in D SC sections 450 μm rostral to the lesion (F). The proportions of slc6a5:GFP⁺ neurons (%) represent the numbers of slc6a5:GFP⁺ neurons normalized to the total numbers of nuclei for each section (G).

Dots represent individual animals from 2 independent clutches. For all quantifications, cross-SC sections 450 μm rostral to the lesion site were quantified. Error bars depict SEM, and statistical significance was determined by one-way ANOVA. Multiple-comparisons p values are shown. *p < 0.05; ns, p > 0.05. Scale bars, 50 μm.

Acknowledgments

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