Figure 7
(A–D) Alterations of E-cadherin expression and different BMP factors in heterozygous and wild-type embryos, respectively: E-cadherin, bmp7, bmp4 and bmp2b. qPCR experiments were performed at 48 hpf in triplicate on cDNA samples derived from n = 30 whole embryos per condition to measure the mRNA level of E-cadherin and BMPs factor in ap1g1+/+ and ap1g1+/−. Gene expression was normalized using rpl13a as a reference gene and expressed as the mRNA fold increased. Data are representative of three replicates and are shown as the mean ± standard deviation; * p < 0.01 Student’s t-test, Statistical analysis was performed with GraphPad Version 8.3.3 GraphPad Software, Inc., Version La Jolla, CA, USA.