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Figure 5
DNA integrity in zebrafish sperm: (A) comet with high DNA fragmentation; (B) comet with low DNA fragmentation; (C) DNA fragmentation of fresh sperm obtained from untreated Tg(ins:nfsb-mCherry) males (n = 4) and metronidazole (Met)-treated males (n = 8) and of cryopreserved sperm from untreated males (n = 5) and Met-treated males (n = 6). The plotted values represent means ± SD. Different letters represent statistical differences between treatments and the asterisk represents a statistical difference between fresh and cryopreserved sperm (independent samples t-test, p < 0.05).
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