Figure 4
Sperm motility parameters of fresh sperm obtained from untreated Tg(ins:nfsb-mCherry) males (n = 5) and metronidazole (Met)-treated males (n = 8) and of cryopreserved sperm from untreated (n = 5) and Met-treated males (n = 10). Sperm was activated, and motility parameters were recorded every 10 s for 1 min in terms of: (A) total motility (%); (B) progressive motility (%); (C) curvilinear velocity (μm/s); (D) straight-line velocity (μm/s), and (E) linearity (%). The plotted values represent means. The dashed lines represent fresh sperm and the continuous lines represent cryopreserved sperm. Sperm from untreated males is represented with a white circle and sperm from Met-treated males is represented with a dark circle.