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FIGURE 4
Quantitative data analysis with pyHeart4Fish. Analysis of treated fish with pyHeart4Fish revealed multiple functional heart phenotypes. (A) The left graph presents the detected heart rate derived from the fast fourier transformation (FFT). The right graph displays the approximate heart size in µm2. (B) Percentual data on the severity of manually assessed pericardial edema in treated Tg(myl7:eGPF)twu34/+ zebrafish. (C) The relative contractility derived from percentual size changes of each heart chamber (left graph). Approximate ejection fraction determined by chamber volume changes (right graph). (D) Mean max. heart chamber size (dilation) is presented on the left, while the mean min. heart chamber size (max. contraction) is displayed on the right. The number of fish analyzed is shown in brackets after the treatment condition. Dotted gray lines indicate z-scores of ±1.96 and statistical significance with an alpha error of 5%.