FIGURE 1

Overview of primary screening (A) High-content screening results for z-normalized heart rate data, obtained by analysis with HeartBeat software (v.2.1). Z-scores above or below ±1.96 are considered significant with a 95% CI. For the primary screen, effect sizes of a 90% CI (±1.64) were considered to reduce false-negative results. The center pie-chart shows the therapeutic classes of the 134 hits (90% CI). The therapeutic classes of the remaining 31 hits after filter application is shown on the right. (B) The structure of selected hit compounds with cardioactive properties. Class = therapeutic class; CNS = central nervous system; EMA = European Medicines Agency; FDA = Food and Drug Administration; HCl = Hydrochloride; OTC = over-the-counter drug; anatomical therapeutic chemical (ATC) classification are displayed (indicated by #) if available.