ZFIN Image: Athapaththu et al., 2022, Fig. 1

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Figure Caption

Fig. 1 Pinostrobin at concentrations of less than 20 μM does not show cytotoxicity. (A) Chemical structure of pinostrobin. (B-F) RAW 264.7 macrophages were treated with 0–40 µM pinostrobin for 2 h, followed by stimulation with 300 ng/mL LPS for 24 h. (B) Cell viability was determined by MTT assay. (B) Morphological images were captured under phase-contrast microscopy. (C) Cell viability was measured using flow cytometry. (D) Viable cell count and (E) dead cell population were obtained from flow cytometric analysis. ^&&&, p < 0.001 vs. untreated cells (Student's t-test); ^#, p < 0.05, ^##, p < 0.01, and ^###, p < 0.001 vs. untreated cells (One-way ANOVA); *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. LPS-treated cells (One-way ANOVA).

Acknowledgments

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