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Fig. 5.
Anti-angiogenic drug SU5416 reduces caudal fin regeneration in aged fish. Caudal fin angiogenesis assay with old turquoise killifish (aged 32 weeks) and control or SU5416 treatment. (A) Microscopy images of caudal fins of turquoise killifish during caudal fin regeneration assay n=6 for DMSO and n=8 for SU5416. Scale bar: 1 mm. (B) Quantification of total regeneration of caudal fin. (C) Quantification of thickness of regenerating area. (D) Measurement of blood vessel density in histological sections. (E,F) qPCR measurements of mRNA expression of vascular biomarkers cd34 (E) and vegfa (F). n=7 for both groups. Statistical analysis in C and D is two-way ANOVA (treatment and time as factors, all time points used in analysis) and in D, E and F Mann–Whitney U-test.