FIGURE 6
Myelination of the visual system. Confocal images of whole mount olig2:EGFP/mbpa:tagRFPt embryos at 5 (a, a?), 7 (b, b?), and 11 (c?) dpf. A 25 ?m thick stack is shown as max intensity projection in the OT area (a, b, and c) and the optic nerve chiasm (a?, b?, and c?). Detection of mbpa:tagRFPt starts at 5 dpf in the ON (a?). The number of mbpa:tagRFPt cells increase significantly from 3?4 cells at 5 dpf to 8?10 cells at 11 dpf (a?, b?, c?, quantified in d). A total of 40% olig2:EGFP colocalize with mbpa:tagRFPt at 5 dpf. This percentage increases to 60% from 5 dpf to 7 dpf (quantified in e). E: eye: L: lens; MBH; midbrain hindbrain boundary; ON: optic nerve; OT: optic tectum; PGZ: proliferative germinal zone; SC: spinal cord. Scale bar: 100 ?m