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Figure 1
A simplified workflow and validation of antibodies by Western blot. (A) A simplified flow chart of antibody preparation. (B) Validation of the antibodies by Western blot. (C) Route diagram of the experimental procedures; the preparation of adhesive coverslips and immunofluorescence are performed in 24-well plates; (D) The schematic showing freezing sample in OCT; (E) The schematic showing coating with PLL; (F) The schematic showing mounting sample.
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Full text @ Front Endocrinol (Lausanne)