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Fig. 6.
Generation and characterization of Df(her4;12) and Df(her2;15) chromosomal deletions. (A,B) Schematic of CRISPR/Cas9 strategy. Scissors, sgRNA positions; square brackets, deletion;>or<signs, 5′-to-3′ orientations of genes; half-arrows, binding sites genotyping primers. For details see Fig. S12. (C-F′) WISH for her4, her15 and neurog1 mRNAs in 96 hpf larvae, genotypes as indicated (C-D′,F,F′ dorsal view; E,E′ midsagittal optical section). n, embryos analyzed. MHB, midbrain-hindbrain boundary; rh, rhombencephalon; tel, telencephalon; th, thalamus. Scale bars: 100 µm.
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