Fig. 3.

her6 and her9 single and double mutants reveal auto- and cross-regulation. (A,B) CRISPR/Cas9 deletion of the bHLH domains in her6 (A) and her9 (B). Straight vertical lines, sgRNA binding sites; scissors, endpoints of the small deletion; half-arrows, genotyping primers. (C-N′) WISH for her6 or her9, genotypes as indicated. (C-N) Lateral single sagittal optical planes. (C′-N′) Dorsal views. n/n indicate numbers of embryos with phenotype shown in panel/total analyzed. (O) qPCR analysis of her6 or her9 expression in her6 and her9 single and double 96 hpf mutants. Data are mean±s.d. *P<0.05, **P<0.01; two-tailed, two-sample equal variance t-test (Table S6). MHB, midbrain-hindbrain boundary; pth, prethalamus; rh, rhombencephalon; tel, telencephalon; th, thalamus. Scale bars: 200 µm.