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Figure 1
Phospho-S1P1 T236 Levels, but not Total S1P1, are Elevated in Human TNBC Cells. (a–c) Relative transcript levels of SPHK1 (a), SPHK2 (b), and S1P1 (c) in human TNBC (n = 41) and non-TNBC samples (n = 385) from a reanalyzed microarray dataset (GSE2034; [24]). (d) Western blot analysis of phospho-S1P1 T236 (P-S1P1 T236), total S1P1, and ACTIN levels in human TNBC (n = 6) and luminal (n = 10) cell lines with MCF10A nontransformed cell line as a control. (e–g) S1P1 (e) and P-S1P1 T236 (f) to ACTIN as well as P-S1P1 T236 to S1P1 ratio (g) in TNBC and luminal breast cancer cell lines. Data indicate mean ± SEM.
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