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Figure 8
Characterization of acute tissue inflammation induced by rSpike in zebrafish male adults. Histopathological analysis of intestine (A) and liver (B) of zebrafish injected with recombinant Spike protein (rSpike) and PBM. The evaluations occurred after 6 and 24 h. All sections were stained with Hematoxylin and Eosin. Scale: 40 μm. Arrows indicate Nv: Normal villi; Cd: dilated vasa chylifer; Li: lymphocytes, Nh: normal hepatocytes; Hv: hyperemia of vessels; Th: turgid hepatocytes; Si: sinusoids; Ne: necrosis; Bh: basophil hepatocytes. (C) Cumulative Kaplan–Meier probability curve indicating the survival rate of zebrafish males submitted to different treatments: PBM (control), injected with recombinant Spike protein (rSpike), and (rSpike + PBM).