Fig. 2

Fig. 2

Treatment with H₂O₂ causes mitochondrial expansion. Representative cells from neuro:mitoGFP larvae at 6 dpf, control (A–C) and H₂O₂‐treated (D–F). (A, D) Maximum intensity projection of neurod:mitoGFP (grey). Scale bar, 5 μm. Paraview 3D representation of the mitochondrial objects (B, E) and the skeletonised network (C, F) following analysis with mitograph. Skeleton segment lengths (μm) are as indicated by colour scale. (Details of analysis pipeline are in the Methods and Fig. S1.) (G–N) Quantification of mitochondrial parameters in islet cells of control versus H₂O₂‐treated larvae. Mitochondrial length (G), number of edges (H), PHI (I) Connectivity Score (J) and Average Degree (K) increased with treatment, number of nodes (L), connected components (M) and average edge length (N) did not significantly change with H₂O₂ treatment. *P < 0.05, **P < 0.01, ns not significant. (Results combined from three independent experiments, control, n = 12; H₂O₂ treated, n = 12).