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FIGURE 2
Acute volume loading protocol. (A) Acute volume loading involved three 30 s periods of 3 μL/min saline injection through the micro-cannula, with a 90 s rest period between each, and measurements taken immediately before (B1-B3) and at the end of load application (S1-S3). (B) The pressure and flow during loading were monitored and recorded by the pressure-flow control software.
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