ZFIN Image: Valcarce et al., 2023, Fig. 7

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Fig. 7

RNA-seq analysis of zebrafish 7-dpf larvae (control and stressed). Stressed progenies (S⁺) were obtained from a control female crossed with a male exposed to the 21-day CS protocol. Control progenies (S^?) derived from a control unexposed male crossed with a control female. A Heatmap generated by unsupervised hierarchical clustering of RNAseq expression z-scores computed for the 1705 differentially expressed genes (DEGs) (cut-off criteria: p. adjusted < 0.0500; |FC| > 1.5) between S^? and S⁺ larvae. The heatmap was generated with the ?pheatmap? R package (https://CRAN.R-project.org/package=pheatmap) (n = 4 progenies/treatment). B Representation of the 1705 DEGs (S⁺ vs S^?) detected by RNA-seq passing the stablished threshold identifying the number of upregulated and downregulated DEGs and classifying them by their FC. C Normalized gene expression obtained in the qPCR validation assay for the RNA-seq. Data in C are shown as mean � SEM (*p < 0.0500; **p < 0.0100)

Acknowledgments

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