ZFIN Image: Begeman et al., 2022, Fig. 6

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Figure Caption

Fig. 6 Adding downstream elements to the P0.8 promoter increases developmental enhancer activity.

(A) Structure of the ZSP synthetic promoter. (B?E) 3-dpf F₀ uninjured heart, eye, fin fold, notochord, and vasculature of ZSP (B), LEN-ZSP (C), 103runx1EN-ZSP (D), and gata2a-i4-ZSP (E) larvae. Arrows indicate EGFP expression. Eyes are demarcated by dashed lines. The percentage of EGFP⁺ ?larvae were compared between larvae harboring each ZSP construct and each corresponding P0.8 construct. (F?H) 6-dpf F₀ regenerating hearts, eyes, and fin folds following injury in ZSP (F), LEN-ZSP (G), and 103runx1EN-ZSP (H) larvae. (I) Table of larvae displaying EGFP expression in each tissue for ZSP alone or when paired with LEN or 103runx1EN. Scale bars: 100 ??m ????, p ?< ?0.0001.

Acknowledgments

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Reprinted from Developmental Biology, 492, Begeman, I.J., Emery, B., Kurth, A., Kang, J., Regeneration and developmental enhancers are differentially compatible with minimal promoters, 47-58, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.