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Fig. 3

ID: ZDB-IMAGE-230403-18
Publication: Liu et al., 2023 - Zebrafish ELL-associated factors Eaf1/2 modulate erythropoiesis via regulating gata1a expression and WNT signaling to facilitate hypoxia tolerance
All Figures: Figures for Liu et al., 2023

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Fig. 3

Effects of eaf1/2 deficiency in erythrocytic-fluorescence transgenic fish. A Gene expressions, eaf1, eaf2, gata1a, hbbe3, olig2 and myod, in drl⁺ or gata1⁺ cells collected from Tg (drl: GFP) (A1) and Tg (gata1a: DsRed) (A2) embryos, respectively. B, C Representative images of Tg (drl: GFP) (B1-B3) and Tg (gata1a: DsRed) embryos (C1-C3) injected with eaf1-MO or eaf2-MO at 24 hpf or 48 hpf, respectively, and quantification of the number of drl⁺ cells (B4) and gata1a⁺ cells (C4), and the GFP (B5) and DsRed (C5) fluorescence intensity in white box. D Gene expressions, eaf1, eaf2, gata1a, hbbe3, olig2 and myod, in drl⁺ or gata1a⁺ cells collected from Tg (drl: GFP) (D1) and Tg (gata1a: DsRed) (D2) embryos injected with eaf1-MO or eaf2-MO, respectively. Each experiment was repeated at least three times with similar results for two or three replicates, and a representative result was shown. B1-B3, C1-C3, lateral view, anterior to the left. *P < .05, **P < .01, ***P < .001. NS, not significant. Scale bar = 100 μm (B1-B3, C1-C3)

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