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Figure 2.
(a) Illustration of the locations of the gRNA designed for targeting the TSS region of the cyp19a1a gene; (b) Methylation levels of individual CpGs in the TSS region of the cyp19a1a gene; (c) The average DNA methylation level of target locus within the dmrt1 TSS region at 24 hpf. (d) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene at 24 hpf. (e) The average DNA methylation level of target locus within dmrt1tss region at 6 hpf. (f) Methylation levels of individual CpGs in the TSS region of the dmrt1 gene at 6 hpf. The asterisk indicates statistical significance. (* p<0.05).
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