Figure 5

Olfactory receptor genes are expressed in the adult zebrafish brain. (A) Schematic drawing of a lateral view of the adult zebrafish brain indicating the anterior–posterior levels (a–c) of the transverse sections in (B) and (C). (B) Schematic image of transverse sections of adult zebrafish brain with different areas labeled in the forebrain (a), midbrain (b) and hindbrain (c) areas. Modified from Wulliman et al.²⁴. (C) Representative transverse sections of adult zebrafish brains processed by in-situ hybridization for or102-3, or103-1, or103-5, or104-1, or105-1, or109-9 and or112-1 show different patterns of expression in distinct brain regions. Olfactory receptor gene transcripts are found in the habenula (red asterisk), preoptic area (blue arrowhead), hypothalamus (black asterisk), ventral thalamus (magenta bracket), diffuse nucleus of the inferior lobe (red arrowhead), optic tectum (black arrow), torus longitudinalis (green arrowhead), valvular cerebellum (orange arrow), eminentia granularis (magenta arrow), interpeduncular nucleus (blue arrow) cerebellum (white asterisk), medulla oblongata (blue asterisk), torus lateralis (magenta arrowhead). Scale bar = 500 μm.