Fig. 5

Fig. 5

Fig. 5. Silica crystals signal through the canonical inflammasome in zebrafish. SiO₂ crystals or PBS were injected in the hindbrain ventricle (HBV) of 2 dpf Tg(lyz:DsRED2) (A) and wild type (B-M) larvae in the presence of either DMSO or the caspase-1 inhibitor VX-765 (VX). Neutrophil recruitment and number were analyzed at 6, 12 and 24 hpi by fluorescence microscopy (A), while the transcript levels of the indicated genes (B-L) were analyzed at 12 hpi by RT-qPCR in larval head and tail. Caspase-1 activity levels were analyzed at 24 hpi in larval head and tail (M). Each dot represents one individual and the mean ± S.E.M. for each group is also shown. One representative RT-qPCR and caspase-1 activity experiment from three biological replicates is shown. P values were calculated using one-way ANOVA and Tukey multiple range test. ns, not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.