Fig. 5

Formation of the distal fin-ray vasculature. (A) Confocal image of the pectoral fin vessels (green) of a 3 dpf Tg(kdrl:egfp)^s843 transgenic zebrafish (other surrounding vessels in gray). Dashed box indicates the approximate area imaged in B-F. (B-F) Confocal image time series of the pectoral fin distal vascular plexus of the same Tg(kdrl:egfp)^s843 transgenic zebrafish as in A, imaged at 3 (B), 4 (C), 5 (D), 6 (E) and 8 (F) dpf. (G) Schematic illustrating the series of events occurring in the distal fin from ∼3 dpf to 18 dpf (up to ∼7.4 mm), encompassing the stages shown in A-L. (H-L) Confocal image time series of the pectoral fin distal vascular plexus of the same Tg(kdrl:mcherry)^y206, Tg(mrc1a:egfp)^y251 double-transgenic larva imaged at 10 (H), 11 (I), 12 (J), 16 (K) and 17 (L) dpf (4.5, 4.8, 5.2, 6.8 and 7.0 mm in length, respectively). See Movie 9 for 3D reconstructions of the image in L. (M) Schematic illustrating the series of events occurring in the distal fin from ∼18 dpf to 22 dpf (7.3 to 9.0 mm), encompassing the stages shown in N-Q. (N-Q) Confocal image time series of the pectoral fin distal vascular plexus of the same Tg(kdrl:mcherry)^y206, Tg(mrc1a:egfp)^y251 double-transgenic larva imaged at 18 (N), 19 (O), 20 (P) and 22 (Q) dpf (7.3, 7.6, 7.9 and 9.0 mm in length, respectively). See Movie 10 for 3D reconstructions of the images in N-Q. White arrows denote the ventral extent of the Tg(mrc1a:egfp)^y251 blood vessel; cyan arrows denote rarified areas of the distal plexus; magenta arrows denote the new venous sprouts that will form the dorsal fin-ray vein. A ‘time series’ of the images in H-Q is shown in Movie 11. Images shown are representative of data collected from 12 separate larvae. Scale bars: 50 μm.