Fig. 2

Fig. 2

Fig. 2. Mirror-biting test and shoaling behavior of zebrafish after treatment with ammonia for 4 h. (A) Schematic illustration of the mirror-biting test. (B) Adult zebrafish were treated with 0, 90, and 180 mg/L (NH₄⁺), and the number of times a fish bit the mirror was counted. Fish treated with 180 mg/L (NH₄⁺) showed a lower mirror-biting number. (C) Schematic illustration of the shoaling behavior test. (D) Adult zebrafish were treated with 0, 90, and 180 mg/L (NH₄⁺), and the average inter-fish distance during shoaling was analyzed. Data are shown as the mean ± SD. * Indicates a significant difference from the control (0 mg/L) by Student’s t-test, p < 0.05. NS, not significant.