ZFIN Image: Li et al., 2023, Figure 8

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Figure Caption

Figure 8

Zebrafish HERC7c is an E3 ligase for the conjugation of ubiquitin rather than ISG15. (A) Schematic diagram of zebrafish HERC7c protein and crucian carp HERC7 protein. (B) Polyubiquitination of zebrafish HERC7c. HEK293T cells plated in 10 cm² dishes overnight were transfected with 5 μg zebrafish HERC7c-HA together with or without 4 μg His-Ub. After 30 h, cells lysates were incubated with Ni²⁺-NTA resin, followed by Western blotting. (C) ISGylation of crucian carp HERC7 but not of zebrafish HERC7c. HEK293T cells plated in 10 cm² dishes were transfected with zebrafish HERC7c-HA or crucian carp HERC7-HA (5 μg each), together with or without 5 μg Flag-ISG15. After 30 h, the cells were collected for Western blotting.

Acknowledgments

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