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Figure 7
Zebrafish HERC7c-mediated degradation of STING, MAVS, and IRF7 was blocked by MG132 but not by chloroquine. EPC cells seeded in a 12-well plate were cotransfected with DrHERC7c-Flag and DrSTING-HA (A), DrMAVS-HA (B), or DrIRF7-HA (C), respectively. After 24 h, the cells were treated with MG132 (final concentration of 10 mM) and chloroquine (final concentration of 25 mM) for another 6 h, followed by Western blotting.
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