ZFIN Image: Li et al., 2023, Figure 3

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Figure Caption

Figure 3

Zebrafish HERC7c suppresses intracellular poly(I:C)-triggered IFN response by luciferase assays. (A) Overexpression of zebrafish HERC7c inhibited fish IFN promoter activation by intracellular poly(I:C). EPC cells were cotransfected with 100 ng CaIFNpro-luc and increasing doses of zebrafish HERC7c for 24 h, followed by transfection with 1 μg /mL poly(I:C). (B,C) Zebrafish HERC7c inhibited mRNA expression of cellular ifn and viperin in EPC cells by intracellular poly(I:C). EPC cells were transfected with 2 μg of zebrafish HERC7c or pcDNA3.1- as control. After 12 h, the cells were transfected again with 1 μg/mL poly(I:C). At 36 h post infection (B) or at different time points (C), cells were sampled for RT-PCR analyses of cellular ifn and mx transcription. Error bars represent SD obtained by measuring each sample in triplicate. (*** p < 0.001, ** p < 0.01, * p < 0.05).

Acknowledgments

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