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Figure 4
In vitro effect of PDS on pre-miR-150 G4 stability. (a) CD melting curves of the RNA oligonucleotide containing the pre-miR-150 PQS in presence of 1 mM K+ and different PDS concentrations. The Tm for 5 and 10 µM PDS could not be calculated due to the absence of a clear melting transition. CD spectra of the RNA oligonucleotide containing the pre-miR-150 PQS folded in presence of 1 mM K+ and incubated with different PDS concentrations at the start ((b), 20 °C) and at the end ((c), 95 °C) of the melting experiments.
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