ZFIN Image: Johnson et al., 2023, Figure 2

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Figure 2

Novel Tank Dive Test. (A) Average of the ratio of time fish spent in the defined zones of interest. The ratio of interest increased after treatment with 5 and 15 mg/L chlordiazepoxide. (B) Average velocity. Fish move significantly faster after treatment with 5 and 15 mg/L chlordiazepoxide. (C) Average immobility. Fish were significantly less immobile after treatment with 5 and 15 mg/L chlordiazepoxide. (D) Representation of the ratio of time spent in the defined zones of interest, velocity, and immobility on PC1 and PC2. (E) PC1 scores. Significant increases in the PC1 scores were detected at 5 and 15 mg/L of chlordiazepoxide. (F) PC2 scores. A significant difference was detected between the 15 mg/L group and the control group. Significant differences are indicated by *(P < 0.05), **(P < 0.01), ***(P < 0.001), and ****(P < 0.0001). When applicable, all data is shown as mean ± S.E.M.

Acknowledgments

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