FIGURE 3

EWSR1 contributes to for Aurora B localization at inner centromere, and prevents its localization at kinetochore during early mitosis. (A). Representative images of Aurora B on pro/metaphase chromosome obtained from control (AUX-) cells that were released from thymidine/nocodazole for 30 min. The Aurora B (Green, top and bottom panel) and CENP-C (kinetochore marker) (Red, top and middle panel) were visualized by immunocytochemistry using anti-Aurora B and anti-CENP-C antibodies. Classification of the localization of Aurora B; Inner Centromere (IC), Kinetochore Proximal Centromere (KPC), Inner Centromere and Kinetochore Proximal Centromere (IC + KPC), and No Signal. Scale bar = 10 um. (B). Quantification of Aurora B localization at IC, KPC, IC + KPC, and No signal between AUX- and AUX + cells (total of 712–732 chromosomes per sample, n = 3 experiments). Values are mean with standard error of the mean (SEM). Two-tailed paired t-test; *p < 0.05, **p < 0.01, NS; Non-Significant.