Fig. 5

miR-223-3p association with zebrafish eye defects. A the different groups were imaged at ×150 magnification, and the eye images were traced using danioscope software (Noldus Technologies, Netherlands) for eye size measurement. Images were obtained using Lumar 12 stereomicroscope (Zeiss Microscopy) and a Nikon camera at ×100 magnification; scale bar, 10 mm. B incubation of the different groups in glucose affected the development of the eyes, as demonstrated by aberrant vasculature nourishment that led to a significant reduction in eye size. The different groups incubated in 2% and 5% glucose showed significantly reduced eye size on day 3. The miR-223-3p mimic had severely impaired eye morphology compared to the controls in the 5% glucose condition. Total number of larvae measured was n = 10 per group. Statistical analysis was conducted using ANOVA Graph Pad version 9.0