IMAGE

Fig 3

ID
ZDB-IMAGE-230305-21
Source
Figures for Song et al., 2023
Image
Figure Caption

Fig 3 The efficiency of knock out at the eomesa1 and eomesa2 loci was evaluated by Sanger sequencing method at 7 days post fertilization.

(a) The measurement procedure of gene editing efficiency includes population editing efficiency (Ep) and individual editing efficiency (Ei) on 7 dpf larvae. (b) Sanger sequencing chromatographs show overlapped peaks in the eomesa1 T2 and eomesa2 T4 sites in targeted individuals, and the ratio of peak heights from these overlapped peaks between WT and mutants implies the knockout efficiency. The red rectangles indicate the target bases.

Acknowledgments
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