Fig. 2

Fig. 2 Regulation of glucose homeostasis and body weight in adult zebrafish. (A) Glucose tolerance in normal-fed lepa^nz301 fish, lepb^nz302 fish, lepr^nz303 fish, and wild-type controls (n = 12). (B) Baseline blood glucose levels of normal-fed and overfed lepa^nz301 fish, lepb^nz302 fish, lepr^nz303 fish, and wild-type controls. ****p < .0001, two-way ANOVA. (C) Glucose tolerance in overfed lepa^nz301 fish, lepb^nz302 fish, lepr^nz303 fish, and wild-type controls (n = 12). (D) Area under the curve of (A) and (C). *p < .05, one-way ANOVA; ^#p < .05, two-way ANOVA. (E) Body weights of lepa^nz301 fish, lepb^nz302 fish, lepr^nz303 fish, and wild-type control on a 6-week normal diet or overfeeding regime. ***p < .001, repeated-measures ANOVA. (F) Standard length of normal-fed and overfed lepa^nz301 fish, lepb^nz302 fish, lepr^nz303 fish, and wild-type controls at 0, 3, and 6 weeks. *p < .05, one-way ANOVA. (G–J) Examples of leptin receptor-deficient and wild-type zebrafish at the start and end of the overfeeding regime