Figure 4

E. piscicida challenge in sting^(−/−) and WT zebrafish. (A) Percent mortality of sting^(−/−) and WT zebrafish post-E. piscicida infection. Two-month-old juvenile zebrafish were wounded, and E. piscicida infection was performed via bath immersion for 5 h. (B–H) Temporal transcription of tbk1, nf-κb, irf3, irf7, ifnphi1, tnfα, and il6 in juvenile sting^(−/−) and WT zebrafish upon E. piscicida infection. ef-1α expression was used as an internal control to analyze relative mRNA expression using the Livak method, and gene expression was normalized to that of the respective PBS control group. The error bars and the asterisk (* p < 0.01, ** p < 0.001) indicate the standard deviation and significantly different transcription levels compared to the respective control, respectively.