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Figure 4

ID

ZDB-IMAGE-230228-356

Antibodies

Ab1-lama1

Publication

Trejo-Reveles et al., 2023 - Identification of Novel Coloboma Candidate Genes through Conserved Gene Expression Analyses across Four Vertebrate Species

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Figures for Trejo-Reveles et al., 2023

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Figure 4

Targeted gene disruption of OFC novel candidates using CRISPR/Cas9 gene targeting in zebrafish embryos. (a) Sanger sequence traces for genomic DNA PCR analysis of CRISPR/Cas9 targeted embryos show successful genetic disruption at the sgRNA site loci for eph3ba, eph3bb, and emx2. PAM sites are indicated. (b) Brightfield microscopy analysis of targeted fish at 3 dpf and 5 dpf compared to uninjected controls and Cas9-only controls. (c) Eye diameter metrics for fish injected with CRISPR/Cas9 at 3 dpf and 5 dpf (**** = p < 0.0001). (d) Confocal analysis of laminin-stained cryosections from gene-edited and control eyes showing persistence of the basement membrane in the optic fissure margin (arrowheads). Colobomas were observed in 27% and 47% of gene-edited embryos analysed for emx2 and ephb3a/b, respectively (n = 15 per target gene).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules