ZFIN Image: Trejo-Reveles et al., 2023, Figure 3

Image

Figure Caption

Figure 3

Spatial gene expression analysis for 10 conserved fissure-enriched genes in the developing chick eye. Fluorescent in situ hybridisation for (a) known coloboma gene targets and (b) OFC candidate genes identified in this study was counterstained with DAPI nuclear stain on chick eyes at HH28, immediately prior to fusion. Note that for EMX2, the OFM region highlights the POM region; expression was not observed in the NR or RPE. In both (a,b), Top: grayscale RNAscope probe signal without DAPI highlighting the mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of the broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules