Figure 1

Figure 1

Transcriptome profiling of chicken optic fissure closure. (a) Schema for segmental dissection of chick OFM tissue at the fusing (HH.St30) and fused (HH.St34) OFC stages, with arrows indicating the limits of the optic fissure margin. (b) Venn diagrams representing the number of DEGs that were (left) significantly upregulated: Fissure expression > Dorsal, or (right) downregulated: Dorsal expression > fissure) between the fusing HH.st30 fissure (blue circle) and fused HH.st34 fissure tissue (clear circle). The number of DEGs that are commonly upregulated/downregulated between the two stages is indicated in the overlapping region. (c) Volcano plot showing differentially expressed genes in the fusing stage HH.st30 OFC transcriptomes. (d) Volcano plot showing differentially expressed genes in the fused stage HH.st34 transcriptomes. Genes with the greatest differential changes are labelled for each graph. (e,f) Plots showing DAVID enrichment analysis outputs for fissure-specific DEGs at the two developmental stages ((e), HH.St30; (f), HH.St34). Values shown are −Log10 (p value). All accompanying enrichment and gene expression analysis data are in Supplemental Table S1.