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Publication: Ghosal et al., 2023 - Embryonic ethanol exposure disrupts craniofacial neuromuscular integration in zebrafish larvae
All Figures: Figures for Ghosal et al., 2023

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FIGURE 3

Ethanol induces ectopic muscle fibers (A) Ventral view of branchial skeleton and muscle in untreated sox10:Kaede;unc:mCherry fish at 4dpf. (D–F) Ethanol-exposed sox10:Kaede;unc:mCherry fish at 4dpf. Arrows show ectopic muscle fiber in D & E. (B) Ectopic muscle fibers significantly increase in the ethanol-exposed fish. (C) Schematic showing locations of ectopic muscle fiber defects observed in the ethanol-exposed fish. (G) Untreated isl1:RFP fish immunolabeled with MF20 (heavy chain of myosin II) in red and Thbs4b (Thrombospondin 4 b) in green. (H, I) MF20 & Thbs4b immunolabeled, ethanol-exposed isl1:RFP fish. Arrows show ectopic muscle fibers lack ectopic tendons. * = p < 0.05. Scale bar = 100 um.

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