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FIGURE 2
Ethanol can have mild effects on the shape of the basihyal cartilage (A) Ventral view of branchial cartilages in untreated (UT) sox10:Kaede fish at 4dpf. (B) Ventral view of branchial cartilages in ethanol (EtOH)-exposed sox10:Kaede fish at 4dpf showing trident-shaped basihyal (indicated by an arrow). (C, D) Schematics showing basihyal shape in untreated (C) and ethanol-exposed fish (D). (E) Number of fish with trident-shaped basihyal increase in the ethanol-exposed group. (F, G) Alcian and alizarin-stained, ethanol-exposed viscerocranium showing normal ossification at 4dpf in AB fish. (H–J) Live alizarin-stained untreated sox10:Kaede fish at 4dpf. (K–M) Live alizarin-stained sox10:Kaede, ethanol-exposed fish at 4dpf showing no ossification defects. BR (branchiostegal ray), CB5 (ceratobranchial 5), CL (cleithrum), D (dentary), JJ (jaw joint), OC (otic capsule), OP (opercle). Scale bar = 100 um.