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Fig. 6
Cold acclimation alleviated cold-induced cell apoptosis in ZF4 cells. Ctr, the control group; Dg, the domesticated group; Ng, the non-domesticated group. (A) Analysis of cell apoptosis by flow cytometry. Apoptosis was evaluated with AnnexinV-FITC/PI staining. (B,C) Apoptotic cells were calculated and graphed according to the flow cytometry results in GraphPad Prism 8 software. The results are presented as the mean ± SD (n = 3); **P < 0.01.
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